X-ray microanalysis with transmission electron microscopy determined presence and movement of tracer (lanthanum chloride) at blood-neuron barrier of Drosophila melanogaster (Diptera : Drosophilidae) larva |
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| Institution: | 1. McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, WI 53706, U.S.A.;2. Department of Entomology, Neuroscience Training Program, University of Wisconsin-Madison, WI 53706, U.S.A.;1. Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA;2. Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA;1. Department of Biology, University of Vermont, Burlington, VT 05405, USA |
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| Abstract: | In studying the larval Drosophila (Diptera : Drosophilidae) blood-brain barrier, it was important to determine if even minute amounts of tracer ultimately seeped through the septate junctions between perineurial cells to reach the neuronal region. Concurrent TEM with X-ray microanalysis was undertaken to resolve that issue. Ultrathin sections of Drosophila nervous tissue in LR White embedment were exposed to ionic tracer (lanthanum chloride) and assayed for presence or absence of lanthanum extracellular to the perineurium and glia making up the nerve sheath. Tracer filled the distal interseptal lattice of pleated sheet-septate junctions, but was contained prior to reaching the proximal paracellular space. No detectable tracer passed through septate junctions to enter the glial-neuronal domain. Based on our present data and the research of others, septate junctions in immature Drosophila are multifunctional structures that enforce spatial relationships between cells, seal intercellular spaces, and control cell proliferation in the epithelia. Septate junctions in Drosophila with the (dlg) gene also exhibit protein homologies to the Z0–1 human tight junction component. |
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