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Unique genetic compartmentalization of the SUF system in cryptophytes and characterization of a SufD mutant in Arabidopsis thaliana
Authors:Hjorth Elmar  Hadfi Katalin  Zauner Stefan  Maier Uwe-G
Affiliation:Cell Biology, Philipps-University Marburg, Karl-von-Frisch Strasse, D-35032 Marburg, Germany.
Abstract:The mobilization of sulfur (SUF) system is one of three systems involved in iron-sulfur cluster biosynthesis and maintenance. In eukaryotes the SUF system is specific for the plastid and therefore of symbiotic origin. Analyses in cryptophytes showed a unique genetic compartmentalization of the SUF system, which evolved by at least two different gene transfer events. We analyzed one of the components, SufD, in the cryptophyte Guillardia theta and in Arabidopsis thaliana. We demonstrated that SufD fulfils house keeping functions during embryogenesis and in adult plants in A. thaliana.
Keywords:ABC, ATP binding cassette   EST, expressed sequence tag   Fe-S, iron-sulfur   GFP, green fluorescent protein   GST, glutathione S-transferase   ISC, iron-sulfur cluster assembly machinery   NAP, non-intrinsic ABC protein   NIF, nitrogen fixation   SUF, mobilization of sulfur   T-DNA, transferred DNA
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