Numerical taxonomy of α-amylase producing Bacillus species |
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| Authors: | I S Pretorius M J de Kock T J Britz H J Potgieter P M Lategan |
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| Institution: | Department of Microbiology, University of the Orange Free State, PO Box 339, Bloemfontein, 9300, Republic of South Africa;*Department of Medical Microbiology, University of the Orange Free State, PO Box 339, Bloemfontein, 9300, Republic of South Africa |
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| Abstract: | A total of 134 α-amylase producing Bacillus isolates and 21 reference strains were divided into 12 groups according to their similarities (% SSM). Phenotypic characteristics determined by the API 20E and API 50CHB galleries, other biochemical tests and morphological characteristics were used for the numerical analysis. The API Computer Service identified 45% of the isolates. The amylase yields of 16 α-amylase hyperproducing (AHP) isolates were compared with those of seven amylolytic reference and type strains. The AHP isolates were related to Bacillus subtilis, B. licheniformis and 'B. amyloliquefaciens' . |
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