支原体肺炎患儿血清中相关炎性因子的表达变化情况及其临床意义探讨

目的：探讨支原体肺炎（Mycoplasma Pneumoniae Pneumonia,MPP）患儿血清中细胞因子IL-8,IL-12的表达水平及hs-CRP、IgG和血清补体（C）的变化及其临床意义。方法：收集MPP患儿50例,分为重症组、轻症组。健康儿童42例作为对照组;用ELISA法测定MPP患儿急性期、恢复期及对照组儿童血清IL-8、IL-12的水平,用血浆蛋白分析仪速率散射比浊法测定hs-CRP,Ig和C含量。结果：在急性期和恢复期MPP患儿血清IL-12含量明显低于正常对照组（P〈0.05）;而血清IL-8含量在急性期明显高于正常对照组（P〈0.01）。重症组患儿血清中IL-12明显低于轻症组,而血清中IL-8较轻症组高（P均〈0.01）。急性期MPP患儿血清IgM,IgG与对照组相比明显升高（P均〈0.01）;而IgA明显降低（P〈0.05）。急性期MPP患儿hs-CRP、C3、C4与对照组比较显著升高（分别为P〈0.01、P〈0.01、P〈0.05）。重症组患儿血清中IgM,IgG与轻症组相比明显升高（P均〈0.01）;IgA与轻症组相比明显降低（P〈0.05）;重症组患儿血清中hs-CRP、C3、C4与轻症组相比明显升高（P均〈0.01）。结论：检测相关血清炎性细胞因子对判定MPP患儿的病情和预后有较高的临床应用价值。

Expressions and Significance of the Inflammatory Factors in Serum of Children with Mycoplasma Pneumoniae Pneumonia

Objective： To detect the levels of IL-8 and IL-12 in cytokine and to observe the the changes of hs-CRP Ig and complement（C） in serum of children with the MPP. Methods： 50 children with MPP were selected and divided into the severe group and the mild group. Another 42 healthy children were chosen to be the control group. Then the levels of IL-8 and IL-12 in the serum of children in the control group and the children with MPP in the acute and recovery phase by means of ELISA. The changes of hs-CRP, lg and C in serum were detected by Plasma protein analyzer and rate nephelometry. Results： In the acute and recovery phase of MPP, the expression of IL-12 was apparently lower than that of the control group（P〈0.05）, while the expression of IL-8 was significantly higher than that of the control group（P〈0.01）. The expression of IL-12 in the serum of the children in the severe group was markedly lower than that of the mild group, while the expression of IL-8 was higher than that of the mild group（P〈0.01）. In the acute phase, the changes of IgM and IgG in the serum of children with MPP were up-regulated and the changes of IgA was down-regulated（P〈0.05）. In the severe phase, the levels of hs-CRP, C3 and C4 in the serum of children with MPP increased when comparing with the control group（P〈0.01）. IgM and IgG in the serum of the acute group significantly increased, and the IgA decreased when comparing with the mild group; hs-CRP, C3 and C4 in the serum of the severe group significantly increased when comparing with the mild group（P〈0.01）. Conclusion： It is indicated that the detection of inflammatory cytokines in the serum of children with MMP could be helpful to the determination and prognosis of the disease.