Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin |
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Authors: | Diane E Cryderman Matthew H Cuaycong Sarah C R Elgin Lori L Wallrath |
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Institution: | (1) Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA, US;(2) Department of Biology, Washington University, St. Louis, MO 63130, USA, US |
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Abstract: | In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect
variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously,
a P-element mobilization screen identified transgenic Drosophila stocks showing PEV of an hsp70-white
+
reporter gene; transgenes in many of these stocks map to the chromocenter of polytene chromosome. A screen at an elevated
temperature identified two stocks that under standard culture temperatures show complete repression of the hsp70-white
+
transgene. The transgenes in both cases map to the chromocenter of polytene chromosomes. Different types of middle repetitive
elements are adjacent to seven pericentric transgenes; unique sequences are adjacent to two of the perimetric transgenes.
All of the transgenes show suppression of PEV in response to a mutation in the gene encoding heterochromatin protein 1 (HP1).
This suppression correlates with a more accessible chromatin structure. The results indicate that a pericentric transgene
showing PEV can be associated with different types of DNA sequences, while maintaining a common association with the chromosomal
protein HP1.
Received: 15 January 1998; in revised form: 27 May 1998 / Accepted: 4 September 1998 |
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