Comparative genomics inferred two distinct populations of piscine pathogenic Streptococcus agalactiae,serotype Ia ST7 and serotype III ST283, in Thailand and Vietnam |
| |
| Authors: | Pattanapon Kayansamruaj Chayanit Soontara Sasimanas Unajak Ha Thanh Dong Channarong Rodkhum Hidehiro Kondo Ikuo Hirono Nontawith Areechon |
| |
| Affiliation: | 1. Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand;2. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand;3. Department of Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand;4. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand;5. Research Unit of Fish Infectious Diseases (RU-FIDs), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand;6. Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan |
| |
| Abstract: | The genomes of Streptococcus agalactiae (group B streptococcus; GBS) collected from diseased fish in Thailand and Vietnam over a nine-year period (2008–2016) were sequenced and compared (n = 21). Based on capsular serotype and multilocus sequence typing (MLST), GBS isolates are divided into 2 groups comprised of i) serotype Ia; sequence type (ST)7 and ii) serotype III; ST283. Population structure inferred by core genome (cg)MLST and Bayesian clustering analysis also strongly indicated distribution of two GBS populations in both Thailand and Vietnam. Deep phylogenetic analysis implied by CRISPR array's spacer diversity was able to cluster GBS isolates according to their temporal and geographic origins, though ST7 has varying CRISPR1-spacer profiles when compared to ST283 strains. Based on overall genotypic features, Thai ST283 strains were closely related to the Singaporean ST283 strain causing foodborne illness in humans in 2015, thus, signifying zoonotic potential of this GBS population in the country. |
| |
| Keywords: | Corresponding author. cgMLST Comparative genomics Fish Pan-genome Population genetics |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
