Vibrational spectroscopy-based quantification of liver steatosis |
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Authors: | E Szafraniec S Tott E Kus D Augustynska A Jasztal A Filipek S Chlopicki M Baranska |
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Institution: | 1. Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland;2. Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland |
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Abstract: | The liver plays a central role in lipid metabolism, and abnormal lipid accumulation in the liver is a key feature of Non-Alcoholic Fatty Liver Disease. In experimental studies, quantification of liver steatosis is commonly based on lipids staining or biochemical analysis. Here, we present a spectroscopic approach for quantitative analysis of the lipid content in the freeze-dried liver. The method is based on vibrational spectroscopy (Raman and infrared) measurements applied for Partial Least Squares (PLS) regression modeling. The obtained PLS models show a good correlation of the spectroscopic data with the reference histological evaluation of steatosis based on Oil Red O (ORO)-stained images of liver cross sections. Vibrational spectroscopy with PLS-based modeling described here represents a useful approach for the fast assessment of the liver steatosis in a small sample of freeze-dried liver tissue. In conclusion, our work demonstrates the easy-to-use method that can be applied in laboratory routine as a beneficial alternative to the established ORO staining. |
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Keywords: | Corresponding author at: Faculty of Chemistry Jagiellonian University Gronostajowa 2 30-387 Krakow Poland Liver steatosis Lipids Vibrational spectroscopy Oil red O staining |
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