Characterization of the seed and leaf lipids of high and low linolenic acid flax genotypes |
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| Authors: | M L Tonnet A G Green |
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| Institution: | 1. Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China;2. College of Agronomy, Gansu Agricultural University, Lanzhou, China;3. College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China;1. Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Department of Forest Production, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;3. Faculty of Technology Management and Business, Universiti Tun Hussien Onn, 86400 Parit Raja, Batu Pahat, Johor, Malaysia;4. Institute for Infrastructure Engineering and Sustainable Management (IIESM), Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia;1. Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea;2. Center for Natural Products Convergence Research, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea;3. Agronomy Department, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, IFAS, PO Box 110300, Gainesville, FL 32611, USA;4. Department of Biotechnology, Graduate School, Korea University, Seoul 02841, Republic of Korea;5. McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, USA;6. Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, USA;7. Clean Energy and Chemical Engineering, Korea University of Science and Technology, Daejeon 34113, Republic of Korea;1. Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, United States;2. Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA 50011, United States |
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| Abstract: | The total seed lipids of four flax (Linum usitatissimum) genotypes, differing markedly in their acyl composition, were extracted and fractionated using column, preparative, and thin-layer chromatography. In the total lipid extract of seeds, the lower linolenate content of the cultivar Glenelg (39.1% compared to that of cv. Croxton (50.5%) was associated with a higher oleate content. Further reductions in linolenate content in the induced mutants of cv. Glenelg, M1722 (17.2%) and "Zero" (1.9%) were accompanied by equivalent increases in linoleate but only minor increases in oleate. Similar changes were observed in the major triacylglycerol fraction of the simple lipids (fatty acid esters of glycerol and sterols), but there was considerable heterogeneity for acyl composition in the minor simple lipid components, including both diacylglycerols and sterol esters, and the complex lipids (glycolipids and phospholipids). The induced mutations substantially reduced linolenate content of all lipid fractions but in no case was it eliminated. Maturation of "Zero" seed at 15/10 degrees C (compared to 24/19 degrees C) increased linoleate and decreased stearate and oleate contents in all lipid fractions. In contrast to seed lipids, the acyl composition of the leaf lipids of the mutant genotypes was the same as those of their parent. |
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