Chlorophyll interference with phytochrome measurement |
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Authors: | Ann M. Jose Daphne Vince-Prue Janet R. Hilton |
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Affiliation: | (1) Department of Botany Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS Reading, UK;(2) Department of Agricultural Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS Reading, UK |
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Abstract: | Measurements of phytochrome by (A725–815 nm) were completely suppressed at chlorophyll concentrations of the order of 20–40 g g-1 f.wt. in vivo and 37 g cm-3 in vitro, and the readings were reduced by 50% at only 12 g cm-3 in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with A measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.Abbreviations Chl chlorophyll - P phytochrome - Pr and Pfr phytochrome in red absorbing and far-red absorbing forms |
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Keywords: | Chlorophyll Phytochrome |
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