| 江浙蝮蛇毒溶血毒素晶体培养及初步晶体学研究 |
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| 引用本文： | 赵克浩,宋时英,孟五一,林政炯,周元聪.江浙蝮蛇毒溶血毒素晶体培养及初步晶体学研究[J].中国生物化学与分子生物学报,1997,13(5):566-569. |
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| 作者姓名： | 赵克浩 宋时英 孟五一 林政炯 周元聪 |
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| 作者单位： | 中国科学院生物物理研究所生物大分子国家重点实验室，中国科学院上海生物化学研究所 |
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| 摘 要： | 从江浙蝮蛇中分离纯化的碱性磷脂酶Ａ２在ｐＨ９．５，０．０５ｍｍｏｌ／ＬＣＨＥＳ缓冲液中，用汽相悬滴扩散的方法，获得了适用于高分辨率Ｘ射线结构分析的单晶．经Ｘ２００Ｂ面探测器分析，表明该晶体属于正交晶系，Ｐ２Ｉ２Ｉ２Ｉ空间群，晶胞参数为ａ＝９７．１３，ｂ＝１０３．６９，ｃ＝２３．２７．并收集了一套衍射数据，独立衍射点数１２００１个，数据完整度为８６．２％，Ｒｍｅｒｇｅ为０．０４５９．最高分辨率达２．０，根据分子量与晶胞体积估算，一个不对称单位含两个分子．
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| 关 键 词： | 碱性磷脂酶A_2 X射线 晶体生长 |
| 收稿时间： | 1997-10-20 |
The Crystallization and Preliminary Crystallorgaphic Analysis of Hemolytic Toxin from the Venom of Agkistrodon halys Pallas |
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| Zhao Ke Hao,Song Shi Ying,Meng Wu Yi,Lin Zheng Jiong.The Crystallization and Preliminary Crystallorgaphic Analysis of Hemolytic Toxin from the Venom of Agkistrodon halys Pallas[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(5):566-569. |
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| Authors: | Zhao Ke Hao Song Shi Ying Meng Wu Yi Lin Zheng Jiong |
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| Institution: | (National Laboratory of Biomacromolecules,Institute of Biophysics, Academia Sinica,Beijing 100101) Zhou Yuan Cong (Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 20 |
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| Abstract: | The basic phospholipase A 2 isolated and purified by chromatography method from the venom of Agkistrodon halys Pallas namely Agkistrodon blomhoffii brevicaudus exhibits strong hemolytic activity. The enzyme has been crystallized by vapour diffusion techniques under CHES buffer,pH9 5,containing 12 mg/ml protein,5%PEG,0 1 mol/L NaCl and 10 mmol/L CaCl 2. Diffraction data of the crystal has been collected up to 2 0 using SIEMENS X 200B area detector. The crystals belong to orthorhombic system with space group P2 l2 l2 l.The parameters of unit cell are a =97 13, b =103 69, c =23 27,Each asymmetric unit probably contains 2 molecules. |
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| Keywords: | Hemolytic toxin Crystallization X ray diffraction |
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