Strain and cultivar specificity in the Agrobacterium-soybean interaction |
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Authors: | Michael C Byrne Raymond E McDonnell Martha S Wright Michael G Carnes |
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Institution: | (1) Monsanto Company, 700 Chesterfield Village Parkway, 63198 Chesterfield, MO, USA;(2) Present address: Genetics Institute, 87 CambridgePark Drive, 02140 Cambridge, MA, USA |
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Abstract: | The response of Glycine max., G. soja and G. canescens genotypes to inoculation with different Agrobacterium strains was assessed. Percent visual tumor formation and tumor size varied widely among species and genotypes. Susceptible genotypes displayed a heightened response to nopaline strains of A. tumefaciens, relative to octopine, agropine, and A. rhizogenes strains. A nopaline strain engineered to contain a chimeric neomycin phosphotransferase II gene conferred kanamycin resistance to soybean tissue at kanamycine levels as high as 300 g/ml. |
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Keywords: | soybean plant transformation Agrobacterium Tiplasmid nopaline |
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