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Nuclear thyroid hormone receptors: evidence for association with nucleolar chromatin.
Authors:R S Gardner
Institution:Department of Internal Medicine University of Iowa and VA Hospitals Iowa City, Iowa 52242 USA
Abstract:When hypothyroid rat liver nuclei labeled in vivo with 125 I]L-triiodothyronine are incubated with micrococcal nuclease, the nuclear chromatin is digested and chromatin particles are released into the medium. The nuclease-treated nuclei contain intact nucleoli and a residual chromatin fraction. When this residual chromatin is purified, it contains only a small percentage of the initial nuclear DNA but is strikingly enriched in 125 I]L-triiodothyronine. This chromatin fraction has many of the characteristics of nucleolar chromatin including a high protein to DNA ratio, an abundance of nonhistone proteins, and a relatively high RNA to DNA ratio. An association of thyroid hormone receptors with a nucleolar component implicates this organelle in the early events of thyroid hormone action.
Keywords:EDTA  disodium ethylenediamine tetraacetate  SDS  sodium dodecyl sulfate  TCA  trichloracetic acid
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