Isolation and characterization of microsatellite markers from reed parrotbill, Paradoxornis heudei (Aves: Timaliidae) |
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| Authors: | Qing Chang Feifei Shen Wenli Xie Baowei Zhang Kaiya Zhou |
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| Institution: | (1) Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China;(2) College of Life Sciences, Anhui University, Hefei, 230039, China |
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| Abstract: | Eleven microsatellite markers were obtained from reed parrotbill, Paradoxornis heudei, using the fast isolation by AFLP of sequences containing repeats (FIASCO) method as part of an effort to compare levels
of genetic diversity in different populations of China. Polymorphism levels ranged form 5 to 11 alleles (mean = 9.27) using
32 reed parrotbills, with the observed heterozygosity ranging from 0.037 to 0.80. Six loci were significant deviated from
HWE and nine loci showed linkage equilibrium. The utility of these loci on two other Passeriformes species, vinous-throated
parrotbill (P. webbianus) and oriental great reed warbler (Acrocephalus orientalis), were also tested. |
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| Keywords: | Paradoxornis heudei Reed parrotbill Microsatellites Isolation Characterization |
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