|
|||||
|
|
Putrescine uptake regulation in response to alpha-difluoromethylornithine treatment in Leishmania infantum promastigotes |
|
| Authors: | Rafael Balaña Fouce Maria I Escribano Jose M Alunda |
| Institution: | (1) Dpto. Fisiología, Farmacología y Toxicología, Ftad. Veterinaria, Universidad de León, León, Spain;(2) Dpto. Fisiología Vegetal, Ftad. Ciencias, Universidad Complutense de Madrid, Madrid, Spain;(3) Dpto. Patología Animal I, Ftad. Veterinaria, Universidad Complutense de Madrid, Spain;(4) Present address: Dpt. Molecular and Cellular Physiology & Pharmacology. The Milton S. Hershey Medical Center, Pennsylvania State College of Medicine, 17033 Hershey, PA, USA;(5) Dpto. Patología Animal I, Facultad de Veterinaria, Universidad Completense, 28040 Madrid, Spain |
| Abstract: | Summary The putrescine uptake/efflux regulation and their regulatory role on intracellular polyamine pools have been studied in the parasitic protozoa Leishmania infantum. Putrescine uptake was age-dependent with maximal values in logarithmic phase promastigotes and minimal in stationary phase. Moreover, putrescine uptake was activated in response to depletion of intracellular polyamines by alpha-difluoromethylornithine (DFMO) — a well known irreversible enzyme-activated inhibitor of ornithine decarboxylase. Kinetic studies of putrescine uptake induction showed a notable rise in Vmax without Km changes, suggesting a de novo synthesis of putrescine carriers. Putrescine uptake was able to replenish polyamine content and also to recover the proliferative rate in cells treated during 24 hours with DFMO. |
| Keywords: | putrescine uptake polyamine levels difluoromethylornithine Leishmania infantum promastigotes |
| 本文献已被 SpringerLink 等数据库收录！ | |