Polarity of DNA entry in transformation of Streptococcus pneumoniae |
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Authors: | Vincent Mé jean Jean-Pierre Claverys |
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Affiliation: | (1) Centre de Recherche de Biochimie et de Génétique Cellulaires du C.N.R.S., Université Paul Sabatier, 118, route de Narbonne, F-31062 Toulouse Cedex, France |
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Abstract: | Summary DNA transport in Streptococcus pneumoniae was studied using donor molecules labelled either at the 3 or at the 5 end, on one strand only. In contrast to 5 end label, 3 end label was not taken up by the cells indicating that entry is a polarized process. Our results together with those of previous studies are consistent with a model for entry in which double-stranded donor DNA is nicked on binding at the cell surface. Entry of a single strand then proceeds linearly from a newly formed 3 end to the extremity of the donor fragment. |
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Keywords: | Bacterial transformation DNA uptake Polarity |
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