嫁接诱导大豆低镉富集性状及遗传稳定性

通过野外小区实验,从8个大豆品种中筛选出2个低镉富集品种作为砧木,2个高镉富集品种作为接穗植物,研究嫁接当代以及接穗子代镉富集性状的变化,探明嫁接诱导镉富集性状变异的机制及其遗传稳定性.结果表明:大豆的镉富集性状表现出显著的品种间差异,以低镉富集品种(铁丰29和东鲜1号)作砧木,可以使接穗大豆植株(青仁黑1号和中黄38...

Genetic stability of low cadmium accumulation induced by grafting in soybean

A field experiment was conducted with two low-cadmium-enriched varieties as rootstocks and two high-cadmium-enriched varieties as scions from eight soybean varieties,with the aim to study the changes in cadmium accumulation of grafted plants and its first filial generation,and to explore the mechanism and genetic stability of the grafting-induced effect. The results showed that cadmium accumulation had significant differences among soybean varieties. By choosing soybeans with low Cd accumulation( TF29 and DX01) as rootstocks,the Cd concentrations of the scions( QRH01 and ZH38) were reduced by 50%-70% through grafting. Results of highthroughput sequencing and qRT-PCR analysis showed a differential expression of related sulfur metabolism genes induced by grafting. Compared to the self-rooted individuals,the expression levels of APX2,PAPRa,SAM1 a and ATPs in grafted plants were down-regulated by 72. 53%,67.62%,42.37% and 75.78% respectively,whereas APK4 was up-regulated to 2.83 times. As a combination with the highest reduction of cadmium content,QRH01-DX01’s first filial generation remained low cadmium accumulation induced by grafting from its parental generation,with a30%-50% reduction compared to the self-rooted one. Our results indicate that grafting can affect cadmium accumulation in soybean shoots by regulating the synthesis and metabolism of sulfur compounds,and that such change can be stably inherited in the filial generation.