Ethanol inhibits hormone stimulated hepatocyte DNA synthesis |
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| Authors: | E A Carter J R Wands |
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| Affiliation: | 1. Department of Medicine, Harvard Medical School, Boston, MA 02114 USA;2. Department of Pediatrics, Harvard Medical School, Boston, MA 02114 USA;3. the Gastrointestinal Laboratory Massachusetts General Hospital, Boston, MA 02114 USA;4. the Pediatric Gastrointestinal and Nutritional Unit, Massachusetts General Hospital, Boston, MA 02114 USA;5. the Shriner Burns Hospital, Boston, MA 02114 USA |
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| Abstract: | Insulin, glucagon, and epidermal growth factor (EGF) addition stimulated DNA synthesis in primary hepatocyte cell cultures prepared from adult rat liver. The addition of ethanol (20-200mM) to the culture medium resulted in a substantial reduction in DNA synthesis as measured by 3H-thymidine incorporation and autoradiography. This effect was specific for differentiated hepatocytes compared to fibroblasts and two other human hepatoma cell lines. These studies demonstrate in a cell culture system that one of the major properties of ethanol is the inhibition of hepatocyte DNA synthesis. |
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