Quantitation of apolipoprotein A-I in pooled human serum by single radial immunodiffusion and sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
| |
Authors: | Y Sakai K Itakura T Kanada N Ebata K Suga H Aikawa K Nakamura T Sata |
| |
Institution: | 2. Tokyo Laboratory, Daiichi Pure Chemicals Co., Ltd., 5-12 Narihira 5-chome, Sumida-ku, Tokyo, 130, Japan;1. The Second Department of Internal Medicine, School of Medicine, Fukuoka University 34, Nanakuma, Jonan-ku, Fukuoka-city, Fukuoka, Japan, 814-01 |
| |
Abstract: | Apolipoprotein A-I was released from human HDL particles by treatment with 8 M urea, and the free apolipoprotein exhibited identical antigenicity and the same low mobility as purified apolipoprotein A-I in electrophoresis. Treatment of serum with 8 M urea enabled enabled quantitation of apolipoprotein A-I by single radial immunodiffusion assay, as judged by comparison with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|