| 人源抗EPO基因工程抗体重链可变区的克隆和鉴定 |
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| 引用本文： | 刘一平,袁斌,邓继先,蒋忠军,程萱,黄翠芬. 人源抗EPO基因工程抗体重链可变区的克隆和鉴定[J]. 生物技术通讯, 1999, 10(1): 1-4 |
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| 作者姓名： | 刘一平 袁斌 邓继先 蒋忠军 程萱 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 摘 要： | 利用噬菌体抗体显示技术筛选 EPO的人源抗体 ,得到了抗 EPO的人源抗体的重链基因。此抗体基因在噬菌体表面呈现的抗体分子具有良好的抗体活性和特异性。为制备完整的、具有更高亲和力的抗体打下了基础。
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| 关 键 词： | 噬菌体显示 EPO 基因工程抗体 |
Clone and characterization of the heavy chain variable region of human anti-EPO genetic antibody |
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| Liu Yiping,Yuan Bin,Deng Jixian,Jiang Zhongjun,Cheng Xuan,Huang Cuifen. Clone and characterization of the heavy chain variable region of human anti-EPO genetic antibody[J]. Letters in Biotechnology, 1999, 10(1): 1-4 |
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| Authors: | Liu Yiping Yuan Bin Deng Jixian Jiang Zhongjun Cheng Xuan Huang Cuifen |
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| Affiliation: | Liu Yiping, Yuan Bin,Deng Jixian, Jiang Zhongjun, Cheng Xuan, Huang Cuifen;(Institute of Biotechnology,Beijing 100071) |
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| Abstract: | We have cloned a gene of the heavy chain variable region of human anti-EPO antibody from a phage antibody library. The antibody fragment displayed on the phage specificlly interacted with EPO molecular, and it did not reacted with other cytokines such as EGF, FGF, NGF, TNF, G-CSF or GM-CSF. |
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| Keywords: | phage display EPO monoclonal antibody |
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