Differential requirement for accessory cells in polyclonal T-cell activation |
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| Authors: | M L Sopori Y L Hurt S Cherian A M Kaplan T Diamantstein |
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| Affiliation: | 1. Department of Medical Microbiology and Immunology, University of Kentucky Medical Center, Lexington, Kentucky 40536-0084 USA;2. The Immunology Research Unit, Klinikum Steglitz, Freie Universitat Berlin, Berlin, West Germany;1. Institute for Clinical Chemistry, University of Heidelberg Medical Faculty Mannheim, D-68167 Mannheim, Germany;2. Aesculabor Hamburg, D-22769 Hamburg, Germany;3. Department of Thoracic and Vascular Surgery, Georg August University of Göttingen, D-37075 Göttingen, Germany;4. Mannheim University of Applied Sciences, D-68163 Mannheim, Germany;5. Department of Surgery, Georg August University of Göttingen, D-37075 Göttingen, Germany;6. Department of Cardiology and Pulmonary Medicine, Georg August University of Göttingen, D-37075 Göttingen, Germany;7. Bioscientia Institute for Medical Diagnostics, D-55218 Ingelheim, Germany;1. Institute for Clinical Chemistry, University of Heidelberg Medical Faculty Mannheim, D-68167 Mannheim, Germany;2. Institute of Transfusion Medicine and Immunology, University of Heidelberg, Medical Faculty Mannheim, D-68167 Mannheim, Germany;3. Aesculabor Hamburg, Hamburg D-22769 Germany;4. Department of Surgery, University of Göttingen, D-37075 Göttingen, Germany;5. Bioscientia Institute for Medical Diagnostics, D-55218 Ingelheim, Germany;1. Institute for Clinical Chemistry, University of Heidelberg Medical Faculty Mannheim, D-68167 Mannheim, Germany;2. Aesculabor Hamburg, D-22769 Hamburg, Germany;3. iRepertoire Inc., Huntsville, AL 35806, USA;4. HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA;5. Department of Surgery, Hospital of Siegen, D-57076 Siegen, Germany;6. Ingenium Digital Diagnostics, D-87662 Kaltental, Germany |
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| Abstract: | Highly purified rat Ia-negative (OX-6-) and Ia-positive (OX-6+) T cells were employed to examine the requirement for accessory cells (AC) and/or soluble factors in the activation of resting T cells with Con A, PHA, sodium periodate, or antigen. A variety of cells were employed as AC, including Ia-positive and Ia-negative macrophages (M phi), gamma-irradiated (2000 rad) or non-irradiated OX-6+ T cells, and several Ia-negative adenovirus-transformed rat embryo fibroblast cell lines. Our results suggested that for the expression of IL-2 receptors (IL-2R) and proliferation of OX-6- T cells in response to Con A, PHA, or antigen, there was an obligatory requirement for the presence of AC which could not be overcome by the addition of IL-1 and/or IL-2. Activation of OX-6- T cells with antigen required the presence of Ia+ AC, while activation with mitogens could be initiated with Ia- AC. M phi were efficient in AC function in all responses tested, while the AC function of OX-6+ T cells (TAPC) proved discriminatory under different conditions. The optimal response to PHA required much higher concentrations of TAPC as AC than for the Con A response. TAPC failed to stimulate sodium periodate-treated T cells under any conditions tested. Furthermore, when TAPC were employed as AC, their antigen-presenting ability was radiosensitive, while their AC function for Con A and PHA was radioresistant. These results suggest that molecules involved in T cell-AC interactions may differ, depending on the source of AC and/or type of the proliferative stimulus provided to T cells. This data has been discussed in the context of T-cell activation. |
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