Antioxidative responses to different altitudes in leaves of alpine plant Polygonum viviparum in summer |
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Authors: | Yuping Wang Wenliang He Huiying Huang Lizhe An Di Wang and Feng Zhang |
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Institution: | (1) School of Life Sciences, Lanzhou University, 730000 Lanzhou, China;(2) Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, College of Agronomy, Gansu Agricultural University, 730070 Lanzhou, China |
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Abstract: | Mountain environmental stresses result in increased formation of hydrogen peroxide (H2O2) and accumulation of malondialdehyde (MDA) in leaves of Polygonum viviparum. The activities of several antioxidative system enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC
1.11.1.6), peroxidase (POD, EC 1.11.1.7), glutathione reductase (GR, EC 1.6.4.2), dehydroascorbate reductase (DHAR, EC 1.8.5.1)
and the contents of several non-enzymatic antioxidants such as reduced form of ascorbate (ASC), dehydroascorbate (DHA), reduced
glutathione (GSH), and oxidized glutathione (GSSG) were investigated in leaves of P. viviparum, which were collected from three altitudes (2,200, 3,200, and 3,900 m) of Tianshan Mountain in China. The activities of these
four antioxidative enzymes were accompanied by increases of H2O2 levels from 2,200 to 3,200 m. However, the activities of CAT and POD were decreased, whereas the activities of SOD and GR
continually increased at 3,900 m. Analyses of isoforms of SOD, CAT, POD, and GR showed that the leaves of P. viviparum exposed different altitude conditions are capable of differentially altering the intensity. Additionally, two new isoforms
of SOD were detected at 3900 m. A continual increase in the ASC, ASC to DHA ratio, GSH and GSH/GSH + GSSG] ratio, and the
activity of DHAR were observed in leaves of P. viviparum with the elevation of altitude. These results suggest that the higher contents of ASC, GSH as well as an increase in reduced
redox state may be essential to antioxidation processes in the leaves of P. viviparum, whereas antioxidant enzymes system is a cofactor in the processes. |
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Keywords: | Altitude Antioxidative responses Polygonum viviparum |
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