|
|||||
|
|
A highly sensitive assay for adenosine triphosphate employing an improved firefly luciferase reagent |
|
| Authors: | W J Simpson † J L Fernandez † J R M Hammond † P S Senior ‡ B J McCarthy ‡ P H Jago § S Sidorowicz § S A A Jassim ¶ S P Denyer ¶ |
| Institution: | †Brewing Research Foundation, Lyttel Hall, Nutfield, Redhill, Surrey RH1 4HY;‡British Textile Technology Group, Wira House, West Park Ring Road, Leeds LS16 6QL;§Water Research Centre, Medmenham, PO Box 16, Marlow, Bucks SL7 2HD;¶Department of Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, UK |
| Abstract: | An improved firefly luciferase reagent allowed reliable detection of as little as 80 fmo1/1 adenosine triphosphate (ATP). Contamination by ATP of the deionized water diluent limited the sensitivity of the assay in some laboratories. The reagent can be used to measure ATP from less than ten bacterial cells if care is taken to eliminate ATP from equipment and reagents. |
| Keywords: | |