大鼠蓝斑核区神经降压素对迷走—加压反应的影响

本文应用放射免疫、核团微量注射及组织荧光分光测定等实验方法,研究大鼠蓝斑核区神经降压素对迷走-加压反应的影响。结果表明:1.电刺激颈迷走神经向中端,孤束核、蓝斑核区和下丘脑中神经降压素免疫活性物的含量明显增高(p<0.05)。2.蓝斑核区注入神经降压素后,刺激颈迷走神经向中端,迷走-加压反应明显减弱(P<0.01),并呈明显的量效依赖关系。3.蓝斑核区注入抗神经降压素血清,迷走-加压反应明显加强(p<0.01)。4.蓝斑核区注入神经降压素后,刺激颈迷走神经向中端,该区去甲肾上腺素含量明显增高(p<0.05)。以上结果提示:内源与外源性神经降压素参与迷走-加压反应的调节过程,并可能与神经降压素引起蓝斑核区去甲肾上腺素含量增加有关。

EFFECT OF NEUROTENSIN IN LOCUS COERULEUS OF RAT ON VAGUS-PRESSOR RESPONSE

The effect of neurotensin(NT)was studied by method of radioimmunoassay,microinjection and spectrophotofluorometry in locus coeruleus of rat on vagus-pressor response.The results were as follows:(1)The contents of neurotensinimmune reactivity(NT-IR)in the nucleus of the tractus solitarius(NTS),LCand hypothalamus were significantly increased by stimulation of the central endof vagi(P<0.05);(2)The vagus-pressor response was significantly weakenedby NT injection into the LC(P<0.01),being dose-dependent;(3)The vagus-pressor response was significantly potentiated by antineurotensin serum injectedinto the LC(P<0.01);(4)The content of noradrenaline(NA)was increased bystimulation of the central end of vagi after microinjection of NT into the LC.Theresults indicate that endogenous and exogenous NT in the locus coeruleus of therat may modulate vagus-pressor response,possibly mediated by the increase inthe content of NA in LC.