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| 甘蔗茎尖培养中减轻酚害 | |
| 引用本文： | 黄诚梅,李杨瑞,叶燕萍. 甘蔗茎尖培养中减轻酚害[J]. 植物生理学通讯, 2004, 40(1): 39-41 |
| 作者姓名： | 黄诚梅 李杨瑞 叶燕萍 |
| 作者单位： | 1. 广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西作物遗传改良生物技术重点开放实验室,南宁,530007;广西大学农学院,南宁,530005 3. 广西大学农学院,南宁,530005 |
| 摘 要： | 甘蔗茎尖组织预先在无菌水中浸泡30 min后,再接种到附加6-BA 2.0 mg·L-1、NAA 0.1 mg·L-1的MS培养基中,可减轻外植体的褐变;茎尖诱导培养基中直接附加适量的活性炭(AC,以0.02%为宜,最高不超过0.05%),减轻茎尖组织酚害的效果最理想.另外,在培养基中附加抗氧化剂(如:聚乙烯吡咯烷酮,PVP),或根据外植体的褐变情况及时转移到新鲜的培养基中,均能减轻甘蔗茎尖组织的酚害. |
| 关 键 词： | 甘蔗 茎尖培养 酚害 |
| 修稿时间： | 2003-03-03 |
Minimizing the Detrimental Effect of Phenol Pollution in Stem Apical Culture of Sugarcane |
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| HUANG Cheng-Mei,,LI Yang-Rui,,YE Yan-PingThe Key Laboratory of Crop Genetic Improvement and Biotechnology of Guangxi,Nanning , College of Agriculture,Guangxi University,Nanning. Minimizing the Detrimental Effect of Phenol Pollution in Stem Apical Culture of Sugarcane[J]. Plant Physiology Communications, 2004, 40(1): 39-41 | |
| Authors: | HUANG Cheng-Mei LI Yang-Rui YE Yan-PingThe Key Laboratory of Crop Genetic Improvement Biotechnology of Guangxi Nanning College of Agriculture Guangxi University Nanning |
| Affiliation: | HUANG Cheng-Mei1,*,LI Yang-Rui1,2,YE Yan-Ping21The Key Laboratory of Crop Genetic Improvement and Biotechnology of Guangxi,Nanning 530007, 2College of Agriculture,Guangxi University,Nanning 530005 |
| Abstract: | The stem apical tissues of sugarcane were pretreated with sterile water for 30 min and inoculated inMS media with 6-BA 2.0 mg.L-1 and NAA 0.1 mg.L-1. The best result of minimizing phenol pollution wasobtained when 0.02% (not higher than 0.05%) active carbon (AC) was directly added into the media. Theantioxidant (i.e. polyvinylpyrrolidone, PVP) was added into the media or the explants were transferred ontofresh media when they became brown. These measures could minimize the phenol pollution. |
| Keywords: | sugarcane apical culture phenol pollution |
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