Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation. |
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Authors: | Ray Dixon, Christina Kennedy, Adá m Kondorosi, Viji Krishnapillai Mike Merrick |
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Affiliation: | (1) A.R.C. Unit of Nitrogen Fixation, University of Sussex, Falmer, BN1 9QJ Brighton, Sussex;(2) Present address: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Pf 521, Hungary;(3) Present address: Department of Genetics, Monash University, 3168 Clayton, Victoria, Australia |
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Abstract: | Summary A series of mutants defective in nitrogen fixation (nif) were isolated in Klebsiella pneunoniae strain M5a1. The nif mutations were either located on plasmid pRD1 or on the K. pneumoniae chromosome. A total of 37 plasmid mutants and 28 chromosomal mutants were employed in complementation tests using the acetylene reduction technique. Most mutants could be assigned to one of seven nif cistrons: nifA, nifB, nifD, nifE, nifF, nifH, and nifK.Complementation analysis of two nif deletion mutants confirmed transductional evidence that these strains carry nifB-A-F deletions. One deletion mutant had, in contrast to previous transductional analysis, a functional nifK cistron and presumably is deleted for nifB-A-F-E.Examination of the biochemical phenotype of several mutants suggests that the nifA product has a regulatory function, and nifK, nifD and nifH are most probably the structural genes for nitrogenase. |
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