He-Ne 激光辐照与UV-B 辐射对小麦幼苗细胞器中Na+/K+-ATP 酶活性的影响

分别采用5 mJ.s^-1.mm^-2 He-Ne激光辐照、10.08 kJ.m^-2.d^-1增强UV-B辐射及二者组合对小麦(Triticum aestivum)晋麦8号(Triticum aestivum ‘Jinmai8’)幼苗进行处理。第5 天开始测定各处理小麦幼苗叶片中线粒体、叶绿体及细胞溶质中Na⁺/K⁺-ATP酶活性的变化。结果表明, 随着处理天数的增加, 小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na⁺/K⁺-ATP酶活性均在第6天下降, 第7天升高, 而后又逐渐下降。在处理的第7天, 仅He-Ne激光辐照可使小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na⁺/K⁺-ATP酶活性升高; 增强UV-B辐射使各细胞器中Na⁺/K⁺-ATP酶活性下降; 复合处理后小麦各细胞器中Na⁺/K⁺-ATP酶活性均高于UV-B单独辐射处理。实验结果表明 , 一定剂量的He-Ne激光辐照能够部分修复UV-B辐射对小麦幼苗细胞器中Na^+/K⁺-ATP酶造成的损伤。

Effects of He-Ne Laser and UV-B Radiation on Na+/K+-ATPase Activity in Organelles of Wheat Seedlings

Seedlings of wheat (Triticum aestivum) were exposed to He-Ne laser irradiation with 5 mJ.s^-1.mm^-2 power density, enhanced UV-B radiation (10.08 kJ.m^-2.d^-1) and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation. From day 5, changes of Na⁺/K⁺-ATPase activity in mitochondria, chloroplasts and cellular solutes were measured to determine the effects of He-Ne laser irradiation and UV-B radiation on wheat. Na⁺/ K⁺-ATPase activity in mitochondria, chloroplasts and cellular solutes of wheat seedlings was reduced at day 6, increased at day 7, then gradually decreased with treatment days . At day 7, He-Ne laser irradiation resulted in increased activity of Na⁺/K⁺-ATPase in mitochondria, chloroplasts and cellular solutes of wheat seedlings . The activity of Na⁺/K⁺-ATPase was reduced under UV-B radiation. The Na⁺/K⁺-ATPase activity in various organelles by combined treatments was greater than the activity with UV-B radiation. All these results suggested that damage to wheat seedlings induced by enhanced UV-B radiation in terms of activity of Na⁺/K⁺-ATPase in various organelles can be repaired in part by He-Ne laser irradiation.