Cytotoxic effector T cells elicited by the killed tumor vaccine differ significantly from the effectors generated during active growth of a murine B-cell lymphoma |
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Authors: | Sampa Santra Ashwini K Sood Swapan K Ghosh |
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Institution: | (1) Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, USA, IN;(2) Department of Molecular Immunology, Roswell Park Memorial Institute, Buffalo, New York 14263, USA, US |
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Abstract: | Active specific immunotherapy of neoplastic diseases is an elusive goal. Using a murine B lymphoma 2C3, we showed that vaccination
with the killed tumor cells effectively induces protective immunity and a cytotoxic T cell (CTL) response. Similar protection,
however, is rarely observed in mice bearing live tumor cells. These animals usually succumb to the progressively growing tumor.
In this study, we inquired whether the splenic CTL induced during tumor progression in mice differ from those evoked by the
killed tumor cells. Here we demonstrate that the CTL generated following vaccination are significantly different from those
induced in the tumor-bearing hosts. Adding to the complexity, the CTL from the early tumor bearers also differ significantly
from those induced at the late stages. These differences are based on their cytotoxic activity, MHC allele specificity, mitogen
responsiveness, cytokine secretion profile and T cell receptor Vβ gene expression. The results clearly indicate that passive
immunization with killed tumor is most effective, possibly because the CTL induced are not subject to the same regulatory
pressure as those induced during active tumor growth. This decreasing effectiveness of CTL could be due to greater variability
in antigenic stimulus, less involvement of innate immunity, changes in cytokine milieu and/or costimulatory factors.
Received: 5 February 1999 / Accepted: 22 June 1999 |
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Keywords: | Cytotoxic T cells Vaccination Cytokine profile B lymphoma TCR Vβ expression |
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