Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate. |
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| Authors: | Z Zhong A Toukdarian D Helinski V Knauf S Sykes J E Wilkinson C O'Bryne T Shea C DeLoughery R Caspi |
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| Institution: | Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0322, USA. |
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| Abstract: | An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results. |
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