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Genome mining for the discovery of new nitrilases in filamentous fungi |
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| Authors: | Ondřej Kaplan Karel Bezouška Anna Malandra Alicja B. Veselá Alena Petříčková Jürgen Felsberg Anna Rinágelová Vladimír Křen Ludmila Martínková |
| Affiliation: | 1.Centre of Biocatalysis and Biotransformation, Institute of Microbiology,Academy of Sciences of the Czech Republic,Prague,Czech Republic;2.Centre for DNA Sequencing, Institute of Microbiology,Academy of Sciences of the Czech Republic,Prague,Czech Republic;3.Department of Biochemistry, Faculty of Science,Charles University in Prague,Prague,Czech Republic;4.Department of Chemistry, Chemical Engineering and Materials,University of L’Aquila,L’Aquila,Italy |
| Abstract: | Purpose of workOur aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. |
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