Evaluation of equilibrium constants from precipitin curves: interaction of alpha-crystallin with an elicited monoclonal antibody |
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| Authors: | D J Winzor A Stevens R C Augusteyn |
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| Institution: | Department of Biochemistry, University of Queensland, St. Lucia, Australia. |
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| Abstract: | A simple procedure, based on the precipitin curve and the antibody:antigen ratios of the precipitates, is described for evaluation of the intrinsic association constant (k) governing the interaction between a multivalent antigen and a bivalent antibody. Its application is illustrated with a study of the interaction between alpha-crystallin and an elicited monoclonal antibody, which is shown to exhibit essentially identical affinities (k = 9 X 10(4) M-1) for the alpha m and alpha c forms of the antigen. |
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