Ion accumulation in bacterial systems. II. Properties of silicate-metabolizing cell-free extracts and particulate fractions from Proteus mirabilis |
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| Authors: | W Heinen |
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| Affiliation: | 1. Poznan University of Technology, Institute of Chemical Technology and Engineering, Berdychowo 4, 60 – 965 Poznan, Poland;2. Poznan University of Technology, Institute of Chemistry and Technical Electrochemistry, Berdychowo 4, 60 – 965 Poznan, Poland;3. Poznan University of Life Sciences, Agronomy Department, Faculty of Agronomy and Bioengineering, Wojska Polskiego 48, 60-627 Poznan, Poland;4. Quantitative Sustainability Assessment Division, Department of Environmental and Resources Engineering, Technical University of Denmark, Produktionstorvet 424, 2800 Kgs. Lyngby, Denmark;5. Poznan University of Life Sciences, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60-627 Poznan, Poland;6. Department IFA-Tulln, Institute of Environmental Biotechnology, BOKU - University of Natural Resources and Life Sciences, Vienna, Konrad-Lorenz-Straße 20, 3430 Tulln, Austria;7. Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research – UFZ, Permoserstraße 15, 04318 Leipzig, Germany;1. College of Chemical and Biological Engineering, College of Earth Science and Engineering, Shandong Provincial Key Laboratory of Depositional Mineralization and Sedimentary Minerals, Shandong University of Science and Technology, Qingdao, 266590, China;2. Laboratory for Marine Mineral Resources, Center for Isotope Geochemistry and Geochronology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China;3. School of Earth Sciences, University of Bristol, Bristol, BS8 1RJ, UK;4. Cabot Institute, University of Bristol, Cantock''s Close, Bristol, BS8 1UJ, UK |
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| Abstract: | The ability of cell-free extracts and participate fractions from Proteus mirabilis to accumulate silicate was found to be independent from previous adaptation of the cells to silicate. Preparations from silicate-adapted material gave 20–30% better results with respect to the amount of silicate accumulated, but responded more sensitively to minor alterations in the external phosphate concentration. The competitive effect of phosphate was found to occur in cell-free extracts as well as in the particulate preparations. The energy-requiring accumulation of silicate can be supported by either substrate-oxidation (NADH, succinate), or ATP cleavage. While silicate-fixation represents an unspecific ion accumulation process, the specific choice of the suitable membrane-bound ions depends on the ADP present in the system. The respiration-dependent but unspecific accumulation process and the highly specific ADP-ATP reaction combined seemed to act as a control mechanism, regulating the alternative stimulation and depression of either process. |
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