Release of 6-keto-prostaglandin F1α and thromboxane B2 from mouse peritoneal macrophages during their adhesion and spreading on a glass surface |
| |
Authors: | Iwao Koyama Haruo Yamagami Toyoyasu Kuwae Munetsugu Kurata |
| |
Institution: | 1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Josai University, 1 - 1 Keyakidai, Sakado, Saitama, 350-02 Japan |
| |
Abstract: | The release of 6-keto-prostaglandin F1α (6KF1α)_and of thromboxane B2 (TXB2) from cells were investigated using mouse peritoneal exudate cells (PECs) and non-cultured peritoneal macrophages. They were prepared by adhesion to glass dishes and incubated for 1 hr at 37°C in 5% Co2 in air. Both the percentage of spreading macrophages and the release of 6KF1α and TXb2 increased in proportion to the incubation time. 6KF1α and TXB2 were released from the macrophages, not from the non-adherent cells. When PECs were incubated in silicon-coated glass dishes, the spreading of macrophages was hardly detected and lower amounts of 6KF1α and TXB2 were released from these cells compared with cells incubated in non-treated glass dishes. These findings suggest that adhesion with the correlated spreading of macrophages on glass dishes serve as a considerable physical factor for the release of 6KF1α and TXB2. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|