Nanopores and nucleic acids: prospects for ultrarapid sequencing |
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Authors: | Deamer D W Akeson M |
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Affiliation: | Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA. deamer@hydrogen.ucsc.edu |
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Abstract: | DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second. |
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