Enzyme-polyelectrolyte noncovalent complexes as catalysts for reactions in binary mixtures of polar organic solvents with water |
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Authors: | Alexander K Gladilin Elena V Kudryashova Alexander V Vakurov Vladimir A Izumrudov Vadim V Mozhaev Andrey V Levashov |
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Institution: | (1) Chemistry Department, Moscow State University, 000958 Moscow, Russia |
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Abstract: | Summary The formation of non-covalent complexes with polyelectrolytes has been suggested to enhance the resistance of enzymes towards inactivation by organic solvents in their homogeneous mixtures with water. Existence of such complexes in water-cosolvent media was proved by experiments with a fluorescence dye, eosin. In the case of catalysis by -chymotrypsin, formation of the complex with polyelectrolytes produced two major eflects: i) considerable increase in enzyme activity at concentrations of ethanol and N,N-dimethylformamide of 10–30 % v/v; ii) conservation of the enzymatic activity at cosolvent concentrations of more than 40% v/v, where the native enzyme is completely inactive. General character of the observed activation and stabilization phenomena was shown by example of several experimental systems. |
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