Histological study of callus formation and root regeneration from mung bean (Vigna radiata W.) |
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Authors: | Jong- Bum Park Kyu- Bae Lee Sukchan Lee |
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Institution: | (1) Department of Life Science, Silla University, 617-736 Busan, Korea;(2) Department of Educational Biology, Chosun University, 501-759 Kwangju, Korea;(3) Department of Genetic Engineering, Sungkyunkwan University, 440-746 Suwon, Korea |
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Abstract: | We have established a reproducible culture system for callus formation and root development from juvenile stem segments of
mung bean(Vigna radiata). In particular, we have studied the influence of plant growth regulators. Induction of calli from young stem explants was
very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. In regenerating adventitious roots
from callus tissues, we found that a combination of 0.75 mg/L NAA, 1.5 mg/L kinetin, and MS salts resulted in 20% efficiency.
Histological examination showed that callus tissues originated from out-growths of the cambium rings through de-novo meristematic
activity. Those rings were localized outside the vascular cambium. Adventitious roots that developed from root primordia originated
from the center of the Callus masses. These primordia produced tracheid-like cells, which then became meristemoid cells for
the cambium. Newly formed adventitious roots had the typical tetrarche actinostele type. |
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Keywords: | meristemoid organogenesis rooting Vigna radiata |
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