Changes in lipid composition during protoplast isolation |
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| Affiliation: | 1. Plant Physiology Division, DSIR, Palmerston North, New Zealand;2. MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824 U.S.A.;1. Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland;2. Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;1. Centro de Investigaciones y Transferencia de Villa María (CONICET-UNVM), Córdoba, Argentina;2. Facultad de Ciencias Médicas, Universidad Nacional de Córdoba (UNC), Córdoba, Argentina;3. Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-UNC, Córdoba, Argentina;4. Instituto de Genética Experimental, Facultad de Ciencias Médicas, Universidad Nacional de Rosario (UNR), Santa Fe, Argentina;1. Department of Chemical Engineering, Faculty of Engineering and Science, Curtin University, Miri, Malaysia;2. Department of Animal Health and Management, Alagappa University, Karaikudi, India;3. Department of Chemistry, Indian Institute of Technology, New Delhi, India;4. School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON, Canada;5. Chemical Engineering Department, University of Tennessee, Chattanooga, TN, United States;1. Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA;2. Department of Physics and Astronomy, Ohio University, Athens, OH 45701, USA;1. Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University, 9 Gronostajowa St., 30-387, Cracow, Poland;2. Polish Academy of Sciences Botanical Garden-Center for Biological Diversity Conservation in Powsin, 2 Prawdziwka St., 02–976, Warsaw, Poland;3. Laboratory of Molecular Biology and Cytometry, UTP University of Science and Technology, 7 Prof. S. Kaliskiego Ave., 85-789, Bydgoszcz, Poland;4. Insitute of Biology, Pedagogical University of Cracow, Podchorazych 2 St., 30-084, Cracow, Poland;5. Department of Plant Ecology, Institute of Botany, Faculty of Biology, Jagiellonian University, 3 Gronostajowa St., 30-387, Cracow, Poland |
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| Abstract: | During the isolation of mesophyll protoplast from leaves of Arabidopsis thaliana (L.) Heynh. the neutral lipids increased from 8% to 17% of the total lipids, while each of the polar lipids decreased by 10–20%. This treatment also induced acyl group migration which was indicated by the transfer of 16:3 from monogalactosyldiacyglycerol to other lipid. Similar results were obtained when leaf slices or vacuum infiltrated leaves were incubated in an aqueous solution containing 0.5 M sorbitol, indicating that the effect is not specifically related to cell wall removal. These observations suggest that the possible deleterious consequences of such lipid changes on the properties of membranes should be considered whenever protoplasts or protoplast-derived organelles are used for physiological studies. |
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