Some properties of starch branching enzyme from indica rice endosperm (Oryza sativa L.) |
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| Institution: | 1. Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, Liaoning, China;2. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3. Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, Guangdong, China;4. Hainan Yazhou Bay Seed Lab/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan, China;1. Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan;2. Rice Quality Research Unit, Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan;3. Bio-oriented Technology Research Advancement Institution, National Agriculture and Food Research Organization, 1-40-2, Nissinmachi, Kita-ku, Saitama, Saitama, 331-8537, Japan;4. Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Yokohama, Kanagawa, 244-0813, Japan;1. Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, Yangzhou, Jiangsu 225009, China;2. The University of Queensland, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD 4072, Australia;3. The University of Queensland, School of Agriculture and Food Science, QLD 4072, Australia;1. The University of Queensland, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, 4072, Australia;2. Arcadia Biosciences, Inc, Davis, CA, 95618, USA;3. Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China;1. Maize Biology and Genetic Laboratory in Northwest Arid Area in China, Ministry of Agriculture, Northwest A&F University, Yangling District, Shaan Xi Province, 712100, China;2. College of Agronomy, Northwest A&F University, Yangling District, Shaan Xi Province, 712100, China;3. Institute of Food Science and Technology (IFST), Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China |
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| Abstract: | Starch branching enzyme (α-1,4-glucan: α-1,4-glucan-6-glycosyl transferase; EC 2.4.1.18) catalyzes the formation of the α-1,6-bond in branched starch molecules such as amylopectin. Some characteristics of starch branching enzyme in rice endosperm (Oryza sativa L.) were determined because of the importance of starch structure for rice quality. Two or three peaks of starch branching enzyme activity were resolved by anion-exchange chromatography of extracts from high amylose rice. The properties of rice starch branching enzyme were similar to those found for the enzyme from other plant sources except for a much lower molecular weight. Rice branching enzyme had an apparent molecular weight of 40 000 as estimated by gel permeation chromatography. Multiple forms of starch branching enzyme could also be resolved in milled rice, suggesting that relationships between starch quality and characteristics of starch branching enzyme could be examined in the mature grain after harvest. |
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