Somaclonal variation and resistance to Verticillium wilt in lucerne,Medicago sativa L., plants regenerated from callus |
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| Affiliation: | 1. College of Nanotechnology, King Mongkut’s Institute of Technology, Ladkrabang, Bangkok, 10520, Thailand;2. Program in Environmental Science and Technology, Faculty of Science and Technology, Pathumwan Institute of Technology, Bangkok, 10330 Thailand;1. Department of Immunology and Microbiology, Childhood Immunology, Department of Pediatrics, University Hospitals Leuven and KU Leuven, Leuven, Belgium;2. Department of Pediatrics, University Hospitals Leuven and KU Leuven, Leuven, Belgium;8. Laboratory Medicine, Experimental Laboratory Immunology, Department of Laboratory Medicine, University Hospitals Leuven and KU Leuven, Leuven, Belgium;3. St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, Rockefeller University, New York, NY;4. Helix, San Carlos, Calif;5. Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Necker Hospital for Sick Children, Paris, France;6. Paris Descartes University, Imagine Institute, Paris, France;7. Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom;9. Paris Descartes University–Sorbonne Paris Cité, Paris, France;10. Study Center for Immunodeficiency, Necker-Enfants Malades Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France;11. Howard Hughes Medical Institute, New York, NY;12. Pediatric Hematology and Immunology Unit, Assistance Publique-Hôpitaux de Paris, Necker Hospital for Sick Children, Paris, France;1. INSERM UMR 1163, Laboratory of Neurogenetics and Neuroinflammation, Paris, France;2. Paris Descartes University, Sorbonne-Paris-Cité, Institut Imagine, Paris, France;3. General Pediatrics, Infectious Disease and Internal Medicine Department, Hôpital Robert Debré, AP-HP, Paris, France;4. Pediatric Hematology-Immunology and Rheumatology Department, Hôpital Necker-Enfants Malades, AP-HP, Paris, France;5. Faculty of Biology, Medicine and Health, School of Biological Sciences, Division of Evolution and Genomic Sciences, University of Manchester, Manchester, United Kingdom;6. UO Pediatria 2 Reumatologia, G. Gaslini, Genova, Italy;7. UO Pneumologia, G. Gaslini, Genova, Italy;8. Department of Paediatric, Pulmonology Emma Children''s Hospital AMC DD, Amsterdam, The Netherlands;9. Department of Radiology, Erasmus Medical Center, Rotterdam, The Netherlands;10. Department of Paediatrics, Respiratory Medicine and Allergology, Erasmus Medical Center-Sophia Children''s Hospital, Rotterdam, The Netherlands;11. Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium;13. Department of Dermatology, University Hospitals Leuven, Leuven, Belgium;15. Clinical Department of Human Genetics, University Hospitals Leuven, Leuven, Belgium;12. Department of Immunology and Microbiology, Childhood Immunology, University of Leuven, Leuven, Belgium;14. Department of Human Genetics, KU Leuven, Leuven, Belgium;p. INSERM UMR 1163, Laboratory of Immunogenetics of Pediatric Autoimmunity, Paris, France;q. Dermatology Department, National Reference Center for Rare Skin Diseases MAGEC, Hôpital Necker-Enfants Malades, Paris, France;r. CNRS UMR7590, Sorbonne Université, Université Pierre et Marie Curie-Paris6–MNHN–IRD–IUC, Paris, France;1. Department of Immunology and Microbiology, Inborn Errors of Immunity, Department of Pediatrics, University Hospitals Leuven and KU Leuven, Leuven, Belgium;2. Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy;3. Pediatric Hematology and Immunology Unit, Necker Hospital for Sick Children, Assistance Publique-Hopitaux de Paris, Paris, France;4. Université de Paris, Paris, France;5. Institut Imagine, Paris, France;6. Institut National de la Santé et de la Recherche Médicale (INSERM) UMR 1163, Paris, France;7. Collège de France, Paris, France;8. Department of Pediatric Allergy and Immunology, and IISGM Gregorio Marañon University Hospital, Madrid, Spain;9. University Hospital La Paz and Lymphocyte Pathophysiology in Immunodeficiencies Group, IdiPAZ Institute for Health Research, Rare Disease Network Research Center (CIBERER), Madrid, Spain;10. Service de Médecine Interne, Centre Hospitalier Universitaire de Nice, Nice, France;11. Pediatric Oncohematology and Bone Marrow Transplantation Unit, Hôpital de Hautepierre, CHRU, Strasbourg, France;12. Division of Hematology/Oncology, Boston Children’s Hospital, Boston, Mass;13. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Mass;14. Division of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, Calif;15. Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, Department of Clinical and Experimental Sciences, University of Brescia an ASST- Spedali Civili of Brescia, Brescia, Italy;p. San Raffaele Telethon Institute for Gene Therapy (TIGET), Pediatric Immunohematology and Bone Marrow Transplantation Unit, IRCCS San Raffaele Scientific Institute Milan, Milan, Italy;q. Vita-Salute San Raffaele University, Milan, Italy;r. Department of Internal Medicine, Division of Clinical Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands;s. Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, University Complutense of Madrid, Madrid, Spain;t. Center for Clinical Immunology, Immunology Group Children’s Hospital Ricardo Gutiérrez, Buenos Aires, Argentina;u. Primary Immunodeficiencies Unit, Pediatrics, Hospital 12 Octubre, Madrid, Spain;v. Research Institute Hospital 12 Octubre (i+12), Madrid, Spain;w. Complutense University School of Medicine, Madrid, Spain;x. Immunology Department, Epsom & St Helier University Hospitals NHS Trust, Carshalton, United Kingdom;y. Laboratory of Clinical Immunology and Microbiology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md;z. Discipline of Clinical Immunology and Allergy, Department of Internal Medicine, University of São Paulo School of Medicine, São Paulo, Brazil;11. Department of Medicine, Division of First Internal Medicine and Center for Immunologic Rare Disease, Ca’ Foncello Treviso Hospital, University of Padua, Padua, Italy;22. Department of Translational Medical Sciences, Center for Basic and Clinical Immunology Research, University of Naples Federico II, Naples, Italy;33. Istituto Molecolare “A Nocivelli,” Department of Experimental and Clinical Sciences, University of Brescia & Asst Spedali civili, Brescia, Italy;44. Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium;55. Laboratory of Immunoregulation and Mucosal Immunology, VIB-UGent Center for Inflammation Research, Ghent, Belgium;66. Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium;77. Division of Allergy & Immunology, Children’s National Hospital, Washington, DC;88. Division of Allergic Diseases and Immunology, Nationwide Children’s Hospital, Columbus, Ohio;99. Department of Pathology and Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio;1010. Division of Allergy and Immunology, Marmara University, Istanbul, Turkey;1111. Isil Berat Barlan Center for Translational Medicine, Istanbul, Turkey;1212. Istanbul Jeffrey Modell Diagnostic and Research Center for Primary Immunodeficiencies, Istanbul, Turkey;1313. Immunodeficiencies Research Unit, National Institute of Pediatrics, Mexico City, Mexico;1414. Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, United Kingdom;1515. Paediatric Immunology and Haematopoietic Stem Cell Transplantation, Great North Children’s Hospital, Newcastle upon Tyne, United Kingdom;pp. Immunodeficiency Centre for Wales, University Hospital of Wales, Cardiff, United Kingdom;qq. Universidad del Desarrollo, Clínica Alemana de Santiago, Santiago de Chile, Chile;rr. French National Reference Center for Primary Immune Deficiencies, Necker University, Paris, France;ss. Department of Clinical Immunology, St-Louis Hospital-AP-HP, Paris, France;tt. Department of Pediatrics, Dr von Hauner Children’s Hospital, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany;uu. Departments of Medicine and Pediatrics, Mount Sinai School of Medicine, New York, NY;vv. Center for Chronic Immunodeficiency, University of Freiburg, Freiburg, Germany;ww. Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany;xx. Division of Allergy Immunology, Department of Pediatrics, The Children’s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pa;yy. Garvan Institute of Medical Research, Darlinghurst, Australia;zz. St Vincent’s Clinical School, UNSW Sydney, Darlinghurst, Australia;111. Hospital Roberto del Rio, Santiago, Chile;1. Division of Pediatric Hemato-Oncology, Department of Pediatrics and Adolescent Medicine, Research Unit for Pediatric Hematology and Immunology, Medical University Graz, Graz, Austria;2. Institute for Immunodeficiency, Center for Chronic Immunodeficiency (CCI), Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany;3. Central Facility Biobanking, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany;4. Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy;5. Great Ormond St Hospital for Children NHS Foundation Trust and UCL Institute of Molecular and Cellular Immunology, Institute of Child Health, London, UK;6. Pediatric Immunology and Infectious Diseases, UMC Utrecht, Utrecht, the Netherlands;7. DZIF, German Center for Infection Research, Satellite Center Freiburg, Freiburg, Germany;8. CIBSS – Centre for Integrative Biological Signalling Studies, Albert-Ludwigs University, Freiburg, Germany;9. RESIST – Cluster of Excellence 2155 to Hanover Medical School, Satellite Center Freiburg, Freiburg, Germany;10. CEREDIH, French National Reference Centre for Primary ImmunoDeficiencies and Pediatric Immuno-Hematology and Rheumatology Unit, Necker-Enfants Malades University Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France;11. Center for Pediatrics and Adolescent Medicine, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany |
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| Abstract: | Somaclonal variation in disease reaction type to infection by the vascular wilt pathogen Verticillium albo-atrum Reinke & Berth was assessed in a population of lucerne plants regenerated from callus lines obtained from a susceptible cultivar. Disease severity in the regenerant population was reduced by comparison with parental controls. Seed progeny and plants recovered via a second tissue culture cycle reverted to mainly susceptible reaction types. In a further experiment a low molecular weight toxic fraction from culture filtrates of the fungus was incorporated into the callus medium prior to regeneration. Toxin treatment reduced the regenerative capacity of callus, and there was little evidence for a higher frequency of wilt resistant plants in populations selected at low toxin concentrations. The results suggest that somaclonal variation as an alternative breeding strategy for disease resistance in lucerne offers no advantages over conventional recurrent selection. |
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