Failure of 2 Hydroxyestradiol to interact with dopamine inhibition of human prolactin secretion in vitro and with dopamine receptors of prolactin-secreting adenomas |
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Authors: | F Peillon A M Brandi D Bression M Le Dafniet J Racadot |
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Institution: | Department of Zoology, University of Maryland College Park, MD 20742, USA |
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Abstract: | The ability of 2-Hydroxyestradiol, a catecholestrogen, and 17 beta Estradiol to interact with the dopamine inhibition of prolactin and with dopamine receptors has been tested on dispersed human prolactin-secreting cells obtained from ten pituitary adenomas. There is a 80% inhibition of prolactin secretion obtained by addition of dopamine in a superfusion system. This inhibition is not affected by preexposure to the steroids, or by their introduction into the perifusion medium. Moreover 2 Hydroxyestradiol and 17 beta Estradiol do not interact with the binding of 3H Domperidone to DA receptors. |
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Keywords: | AIA allylisopropylacetamide ALA delta-aminolevulinic acid BSA bovine serum albumin PMSF phenylmethylsulfonylfluoride SDS sodium dodecyl sulfate |
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