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Development ofgusA reporter gene constructs for cereal transformation: Availability of plant transformation vectors from the CAMBIA Molecular Genetic Resource Service |
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| Authors: | David McElroy Douglas A Chamberlain Eunpyo Moon Kate J Wilson |
| Institution: | (1) Division of Plant Industry, CSIRO, GPO Box 1600, 2601 Canberra, ACT, Australia;(2) Department of Biology, Ajou University, Suwon, Republic of Korea;(3) Center for the Application of Molecular Biology to International Agriculture, CAMBIA, GPO Box 3200, 2601 Canberra, ACT, Australia;(4) Present address: USDA/ARS Plant Gene Expression Center, 800 Buchanan Street, 94710 Albany, CA, USA;(5) Present address: Centre for Molecular Biotechnology, Queensland University of Technology, 4060 Brisbane, QLD, Australia |
| Abstract: | The use of reporter genes to characterise sequence elements that act to regulate gene expression in transgenic plants has been vital to the development of foreign gene expression strategies for use in cereal transformation. ThegusA locus ofEscherichia coli, which encodes the enzyme -glucuronidase (GUS), is by far the most popular reporter gene used in plant transformation. In this paper we extend the utility of the GUS reporter gene system in cereal transformation by describing and evaluating a number of novel constructs suitable for use in direct gene transfer experiments. These plasmids are all available from the Molecular Genetic Resource Service of the Center for the Application of Molecular Biology to International Agriculture. |
| Keywords: | reporter genes expression vectors cereal transformation |
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