DNA recovery and direct detection of Tn5 sequences from soil |
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Authors: | Sonja Selenska W Klingmüller |
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Institution: | Department of Genetics, University of Bayreuth, Germany. |
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Abstract: | Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed. |
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