Improvement of seed bio-priming of oilseed rape (Brassica napus ssp. oleifera) with Serratia plymuthica and Pseudomonas chlororaphis |
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Authors: | Ruba Abuamsha Mazen Salman |
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Institution: | Institute for Phytopathology, Department of Biotechnology and Biological Control , Christian-Albrechts-University , Hermann Rodewald Str. 9, 24118 , Kiel , Germany |
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Abstract: | Seed bio-priming of oilseed rape (Brassica napus) with the antagonistic rhizobacteria Serratia plymuthica and Pseudomonas chlororaphis was improved. With the imbibition of water, bacteria are transported into the seed where they survive better. To obtain a minimum bacterial density in the seed of log10 5 colony-forming-units (CFUs) seed?1, the bacterial density in the bio-priming suspension should be >log10 9 CFUs mL–1 for S. plymuthica and >log10 8 CFUs mL–1 for P. chlororaphis. Priming duration was reduced from 12 to 2 h for S. plymuthica and 4 h for P. chlororaphis. Among other priming solutions tested, the addition of MgSO4 best supported establishment in the seeds and also improved germination. The optimal bio-priming temperature for S. plymuthica is 28°C and for P. chlororaphis 22°C. Survival of the bacteria inside the seeds was moderately improved by storage at low temperature but considerably prolonged by storage under anaerobic conditions. P. chlororaphis survived significantly longer than S. plymuthica. |
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Keywords: | seed treatment germination biological control survival storage conditions |
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