Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies |
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Authors: | Ye LiBin Zhang JingSong Ye XiJun Tang QingJiu Liu YanFang Gong ChunYu Du XiuJui Pan YingJie |
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Institution: | College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. |
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Abstract: | A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2x10(4)Da (determined by HPLC), and consisted of approximately 90% carbohydrate and approximately 8% protein as determined using the phenol-sulfuric acid method and the BCA protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of D-Glc, L-Fuc, and D-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure-activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using 1H and 13C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC-MS of methylated derivatives, and shown to consist of repeating units with the following structure: Formula: see text]. |
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Keywords: | Ganoderma lucidum Glycopeptide NMR spectroscopy Polysaccharide moiety Structural analysis |
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