| RAPD分析在绢丝昆虫亲缘关系研究中的应用I.蓖麻蚕品种间的遗传差异 |
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| 引用本文： | 左正宏,桂慕燕,王学民,陈元霖. RAPD分析在绢丝昆虫亲缘关系研究中的应用I.蓖麻蚕品种间的遗传差异[J]. 遗传, 2001, 23(2): 128-130 |
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| 作者姓名： | 左正宏 桂慕燕 王学民 陈元霖 |
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| 作者单位： | 1.湖南省岳阳卫生学校，岳阳 4140002.福建省厦门大学生物学系，厦门 3610051.Yueyang Heath School of Hunan,Yueyang 414000;2.Dept.of Bio.Xiamen University,Xiamen 361005,China |
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| 基金项目： | 国家自然科学基金资助项目（批准号39870410） |
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| 摘 要： | 用RAPD技术对蓖麻蚕基因组DNA进行多态性研究，分析了5个蓖麻蚕品种间的遗传差异。结果表明，所采用的40个随机引物中，有27个引物扩增谱带清晰且重复性较好，扩增总片段数达243个，单个引物的扩增片段数在4～17之间，平均为9条，片段大小在0.33～3.0kb之间。不同蓖麻蚕品种间的遗传距离(D)在0.0683～0.1603之间，根据D值，由UPGMA聚类分析软件绘制了它们的聚类分子树。Abstract：Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among eri sickworm. The genetic variance of five erisickworm was studied. The result showed that: 27 of 40 arbitrary primers could amplify clearly with repeatable bands.243 fragments were obtained.Each primer gave 4～17 bands and the average was 9.The length of the band was 0.33～3.0kb. The genetic distance (D) value between different breeds of Eri Silkworm was 0.0683～0.1603. The D value was used to construct a dendrogram by UPGMA.
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| 关 键 词： | RAPD 品种差异 Keywords eri silkworm RAPD 蓖麻蚕 |
| 文章编号： | 0253-9772(2001)02-0128-03 |
| 修稿时间： | 2000-04-04 |
Application of RAPD Technique in Genetic Relationship Study of Silk Insect I. Genetic Variance in Eri Silkworm |
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| ZUO Zheng-hong,GUI Mu-yan,WANG Xue-min,CHEN Yuan-lin. Application of RAPD Technique in Genetic Relationship Study of Silk Insect I. Genetic Variance in Eri Silkworm[J]. Hereditas, 2001, 23(2): 128-130 |
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| Authors: | ZUO Zheng-hong GUI Mu-yan WANG Xue-min CHEN Yuan-lin |
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| Abstract: | Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among eri sickworm. The genetic variance of five erisickworm was studied. The result showed that: 27 of 40 arbitrary primers could amplify clearly with repeatable bands.243 fragments were obtained.Each primer gave 4～17 bands and the average was 9.The length of the band was 0.33～3.0kb. The genetic distance (D) value between different breeds of Eri Silkworm was 0.0683～0.1603. The D value was used to construct a dendrogram by UPGMA. |
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| Keywords: | eri silkworm RAPD genetic variance |
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