Chaperone-based procedure to increase yields of soluble recombinant proteins produced in <Emphasis Type="Italic">E. coli</Emphasis>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

Authors:	Ario de Marco Elke Deuerling Axel Mogk Toshifumi Tomoyasu Bernd Bukau

Affiliation:	1.EMBL Heidelberg,Heidelberg,Germany;2.IFOM-IEO Campus for Oncogenomics,Milano,Italy;3.ZMBH, Universit?t Heidelberg,Heidelberg,Germany;4.Department of Microbiology and Molecular Genetics, Graduate School of Pharmaceutical Sciences,Chiba University,Chiba,Japan

Abstract:	Background The overproduction of recombinant proteins in host cells often leads to their misfolding and aggregation. Previous attempts to increase the solubility of recombinant proteins by co-overproduction of individual chaperones were only partially successful. We now assessed the effects of combined overproduction of the functionally cooperating chaperone network of the E. coli cytosol on the solubility of recombinant proteins.

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