| 密枝瑚菌多糖提取工艺参数优化 |
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| 引用本文： | 苏凤贤,杨全文,李彩霞,李鹏. 密枝瑚菌多糖提取工艺参数优化[J]. 天然产物研究与开发, 2011, 23(4): 751-754 |
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| 作者姓名： | 苏凤贤 杨全文 李彩霞 李鹏 |
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| 作者单位： | 河西学院农业与生物技术学院,张掖,734000 |
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| 摘 要： | 本文对密枝瑚菌多糖的测定方法和提取工艺参数进行了研究。结果表明,采用硫酸苯酚法测定密枝瑚菌多糖方法简便、准确、灵敏度高、重现性好。在此基础上采取单因素实验和正交分析,得到密枝瑚菌多糖最佳提取工艺参数为料液比1∶20,提取时间3 h,提取温度90℃。在此条件下,多糖提取率为2.23%。
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| 关 键 词： | 密枝瑚菌 多糖 提取工艺参数 |
Parameter Optimization of Extraction Technology of Polysaccharides from Ramaria stricta |
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| SU Feng-xian,YANG Quan-wen,LI Cai-xia,LI Peng. Parameter Optimization of Extraction Technology of Polysaccharides from Ramaria stricta[J]. Natural Product Research and Development, 2011, 23(4): 751-754 |
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| Authors: | SU Feng-xian YANG Quan-wen LI Cai-xia LI Peng |
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| Affiliation: | SU Feng-xian,YANG Quan-wen,LI Cai-xia,LI Peng College of Agriculture and Biotechnology,Hexi University,Zhang ye 734000,China |
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| Abstract: | The extraction technological parameters and measuring method of polysaccharides in Ramaria stricta(Pers.:Fr.) Quél.were investigated.The results showed that the method of phenol-sulfurc acid was simple,accurate,high sensitivity,and good reproducibility for determining the content of polysaccharides in R.stricta.Based on the results of the single factor and orthogonal experiments,the optimum extraction condition for polysaccharides was obtained as follows:solid-liquid ratio 1∶ 20,temperature 90 ℃,and extraction times 3.0 h.Under these conditions,the extraction rate of polysaccharides from R.stricta was 2.23%. |
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| Keywords: | Ramaria stricta polysaccharides extraction technological parameter |
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