高压脉冲电场法快速提取北五味子中五味子醇甲的工艺研究

本文采用正交实验优化提取方法,HPLC法测定五味子醇甲含量,对五味子的快速提取工艺方法进行了研究。结果表明:以80%乙醇作为提取液,电场强度10 kv/cm,脉冲数为10,料液比1∶10为最佳提取工艺;影响因素的大小依次为:电场强度、料液比、脉冲数。此法提取五味子中的五味子醇甲,提取率可达0.76%,得膏率达27.886%。本提取工艺可行,经济、省时、回收率高。

Rapid Extracting Technology for Schisandrol A from Schisandra chinensis by High Voltage Pulsed Electric Field Method

The rapid extracting technology for schisandrol A from Schisandra chinensis was investigated by using orthogonal experimental design,and the content of schisandrol A was detected by HPLC.The optimum extracting technology was obtained as follows: 80% ethanol as for the extract liquid,10 kv/cm electric field intensity,impulsion frequency of 10,and 1∶ 10 as the ratio for material and liquid.The effect levels of these factors successively were electric field intensity,the ratio for material and liquid,and impulsion frequency.The content of schisandrol A was 0.76% in S.chinensis,and the yield of extract was 27.886%.The extracting technology is feasible,economic with high recovery ratio and saving time in the process.