| 非洲山毛豆幼茎愈伤组织培养及其鱼藤酮含量检测 |
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| 引用本文： | 吴玉东,冯敏英,陆慧,徐汉虹.非洲山毛豆幼茎愈伤组织培养及其鱼藤酮含量检测[J].天然产物研究与开发,2011,23(5):889-893. |
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| 作者姓名： | 吴玉东 冯敏英 陆慧 徐汉虹 |
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| 作者单位： | 华南农业大学天然农药与化学生物学教育部重点实验室,广州,510642 |
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| 基金项目： | 教育部产学研结合项目(2006D90204003); 广东省产学研结合项目(cgzh2d0504) |
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| 摘 要： | 以MS为基本培养基,以非洲山毛豆幼茎为外植体进行培养,探索2,4-D质量浓度与不同浓度的激素组合对愈伤组织诱导的影响,对最佳组合培养基不同培养时间的愈伤组织中鱼藤酮含量进行HPLC测定。实验结果表明以1.00 mg/L 2,4-D和0.70 mg/L 6-BA组合的MS培养基诱导效果最佳,其诱导的愈伤组织分别培养30、60 d和90 d后鱼藤酮的含量分别为干愈伤组织的0.0013%、0.016%和0.06%,非洲山毛豆幼茎愈伤组织中的鱼藤酮含量随培养时间的延长而提高,统计分析显示差异显著。
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| 关 键 词： | 非洲山毛豆 愈伤组织 激素 鱼藤酮 HPLC 生物农药 |
Callus Culture of Tephrosia vogelii and Content Detection of Rotenone |
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| WU Yu-dong,FENG Min-ying,LU Hui,XU Han-hong.Callus Culture of Tephrosia vogelii and Content Detection of Rotenone[J].Natural Product Research and Development,2011,23(5):889-893. |
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| Authors: | WU Yu-dong FENG Min-ying LU Hui XU Han-hong |
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| Institution: | WU Yu-dong,FENG Min-ying,LU Hui,XU Han-hong Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | The effects of different concentrations of 2,4-D and different hormones in combination on the callus induction explored by cultivating explants of the young stem Tephrosia vogelii using MS medium.The contents of rotenone in different culture time of the callus cultivating by optimum combined medium were determined by HPLC.The callus induction obtained optimum effects by 1.00 mg/L 2,4-D and 0.70 mg/L 6-BA combinations of MS medium,and the rotenone contents of the callus after induced cultivation for 30 d,60 ... |
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| Keywords: | Tephrosia vogelii callus hormone rotenone HPLC biopesticide |
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