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Isolation of 12 microsatellite loci,using an enrichment protocol,in the phytopathogenic fungus Puccinia triticina
Authors:X Duan  J Enjalbert  D Vautrin  M Solignac  T Giraud
Abstract:We report the characterization of 12 polymorphic microsatellite markers in the biotrophic fungus Puccinia triticina, the causal agent of leaf rust on wheat. An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with 15 European isolates. Significant level of cross‐amplification (44% of the loci) was found in P. striiformis.
Keywords:basidiomycota  biotrophic fungus  dikaryote  leaf rust  microsatellite enriched library  Puccinia triticina
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