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New microsatellite markers for the snow crab Chionoecetes opilio (Brachyura: Majidae)
Authors:O Puebla   Parent  J‐M Svigny
Institution:O. Puebla,É. Parent,J.‐M. Sévigny
Abstract:Five microsatellite markers were developed for the snow crab (Chionoecetes opilio) and polymerase chain reaction conditions and/or primer sequences of three previously developed microsatellites were adapted for fluorescent labelling analysis. All loci were analysed in 449 individuals from 11 sampling sites in the northwest Atlantic. The high degree of polymorphism exhibited by these microsatellites (mean of 34 alleles per locus and mean observed heterozygosity of 0.76) suggests that they will be suitable for spatial and temporal genetic analysis of C. opilio populations.
Keywords:Chionoecetes opilio  fluorescent labelling  Majidae  microsatellites  polymerase chain reaction  stutter
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